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AWS Elastic Beanstalk
Developer Guide (API Version 2010-12-01)

Elastic Load Balancer Has Zero Healthy Instances

"Elastic Load Balancer awseb-<yourapp> Has Zero Healthy Instances"

When the health status of all of your instances changes from green to red, Elastic Beanstalk alerts you that your application has become unavailable. Make sure that your application’s health check URL exists. For example, if Elastic Beanstalk makes a health check request to http://healthcheckrocks.elasticbeanstalk.com:80/myapp/index.jsp, ensure that /myapp/index.jsp exists and is accessible. Similarly, for PHP, if you have http://healthcheckrocks.elasticbeanstalk.com:80/myapp/index.php, make sure that /myapp/index.php exists and is accessible. For ASP.NET, if you have http://healthcheckrocks.elasticbeanstalk.com:80/myapp/default.aspx, make sure that /myapp/default.aspx exists and is accessible.