Delete a target group for your Gateway Load Balancer
You can delete a target group if it is not referenced by the forward actions of any listener rules. Deleting a target group does not affect the targets registered with the target group. If you no longer need a registered EC2 instance, you can stop or terminate it.
To delete a target group using the console
Open the Amazon EC2 console at https://console.aws.amazon.com/ec2/
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In the navigation pane, under Load Balancing, choose Target Groups.
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Select the target group and choose Actions, Delete.
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When prompted for confirmation, choose Yes, delete.
To delete a target group using the AWS CLI
Use the delete-target-group command.