If you no longer want to have an instance attached to your Amazon Lightsail load balancer, you can detach it. When you detach a Lightsail instance from a load balancer, we wait until the specified instances are no longer needed before detaching.
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In the left navigation pane, choose Networking.
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Choose the load balancer you want to manage.
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On the Target instances tab, choose Detach next to the load balancer you want to detach.